Progesterone Breeding Information
The following information is for the serious breeder. Some dogs have a problem breeding. To help determine the exact day a bitch should be bred, the following chart may be referred to. This is information that was developed by Shirley Johnston while she taught at the University of Minnesota.
The system requires blood to be drawn from the bitch and sent to a lab that can check the progesterone level of the blood. For this to be effective, the breeder needs to find a lab that can give the results on the test back the day after the blood is drawn. This is not a cheap process. It can cost up to $85.00 per test at the Vet - and you may need several tests.
You can purchase your own test kits from a company called Camelot Farms they sell a product called "PreMate" tests kits. Its comes from a company called Biovet in Canada that distributes it but Camelot Farms and Pinnacle Pet Supply sell it in the states. Years ago these kits were not very good - I understand current tests are very good.
I have talked to some vet friends and this preMate is supposed to be right on. It's 10 tests for $99.99 plus a free instructional video with the first order.
This does not include the vets time.
In our kennel, we draw the blood ourselves. If you are going to do any amount of breeding, I recommend that you ask your vet to show you how to do this.
The early readings are: .1 - .2 .3
Start to draw blood on the 7th day.
They can go back down to .2 or even .1 - this depends on the time of day the blood was drawn.
When the LH surge goes over 1.0 to 1.9 - you breed in 5 to 6 days.
If the 5th day seems a little early - you breed on the 6th and 7th day - then skip a day and breed again.
If you draw blood and the level is .2 or .3 - then wait 3 days and draw blood again.
If you draw blood and the level is .7 or .8 - then you do it every other day
Once you start to see the level at:
2.0 to 2.9 ng/ml = breed in 4 to 5 days
3.0 to 3.9 ng/ml = breed in 3 to 4 days
4.0 to 8 .0 ng/ml = breed today or tomorrow - this is where the LH Surge Occurs.
Over 8.0 ng/ml with no white sells - then the bitch has ovulated
When you hit the level above 4.0 ng/mL this is day 1 of ovulation. Then it takes approximately 48 hours for the ovas to mature enough to be fertelized.....then they can be fertelized for approximately another 48 hours.
When you breed - you breed 2 days in a row - then skip a day and breed another time.
When you have bitches that do not swell up or have a lot of discharge - then check their thyroid level, and they may have to go on thyroid pills.
I would be interested in hearing from any vet that does not agree with these numbers or from people that have used this system and what the results were.