Well, I remain interested in this problem. Here's the results of an evening of web-research.
Latex is the milky sap of some plants. Rubber trees, of course, but also poppies, dandilions, and lettuce, among many others. On exposure to air, latex coagulates, sort of like a blood clot or scab. Latex contains proteins, alkaloids, starches, sugars, oils, tannins, resins, and gums. Latex performs a defensive function for the plant. It's taste can be bitter, discouraging animals from eating the plant. Among it's proteins is chitinase, which breaks down chitin, a key chemical component of the cell walls of fungi and insects. And shellfish.
Caoutchouc is a term referring to natural rubber, and most web sites about it seem to be in French. Synthetic rubber is formed by polymerization of chloroprene (Neoprene) or ethylene chloride and sodium polysulphide (Thiokol). Modern rubber is about half natural and half synthetic.
Latex is made into useful items by removal of water and vulcanization. This hardens rubber and makes it more elastic. It involves heating the rubber and adding sulfur (Charles Goodyear 1839).
At the end of the manufacturing process of natural rubber, proteolytic enzymes migrate to the surface. These are removed by traditional water leaching. One site I looked at described latex matresses used in Europe. These are washed after manufacturing to get rid of excess soaps and proteins.
Now, I don't know why anyone would say use of latex to collect stud dogs is verboten. Johnston's
Canine and Feline Theriogenology shows a latex cone for collection of canine semen and Leerburg's video "Artificially Inseminate Your Dogs" uses the cone. These cones are sold on the web (vetinaryconcepts and lambriarvet). Maybe they are pre-washed. Maybe they are not the same type of latex as the sheath I was using.
On the other hand, it's certainly possible to find web sites that describe sperm immobilization with latex or voice other notes of caution.
But, I used a sheath without prewashing, and I always used a new sheath with each collection. I must assume the "proteolytic enzymes" or "soaps and proteins" on the surface of the latex immobilized the sperm or were spermicidal. Although with synthetic latex, I don't know where proteins would come from, but soap could be toxic enough, I suppose.
Live and learn. But man it bites. <img src="/ubbthreads/images/graemlins/frown.gif" alt="" />
Next step: thoroughly wash sheath and collect my boy, examine ejaculate microscopically, go from there. I don't know that this poor dog will ever breed and tie normally, after I've collected him so successfully. After the other dogs go out, he lies on his back and begs me to rub his stomach. Or climbs into my lap and leans back. I think he wants to be collected.